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Furthermore, the stretching of flexible linkers during the tip-retraction provides a typical non-linear trend in force curves, starting and ending at the zero deflection line, which could help to discriminate specific unbinding events from unspecific ones

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doi: 10.1002/hep.28251 68 ZhangYTanHDanielsJDZandkarimiFLiuHBrownLMet al

Methods EV-F3 production and purification BHK-21 cells were grown to 80% confluency before infection with EV-F3 virus stocks in Glasgow modified Eagles medium (G-MEM BHK-21, Gibco), supplemented with 2% fetal horse serum (Thermo Fisher Scientific) and minimal essential medium non-essential amino acids (MEM NEAA, Gibco)