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Intracellular ROS levels were detected by incubating cells with 10 M CM-DCFDA for 30 minutes, then analyzed by Accuri-C6 flow cytometry

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However, this must be done with close monitoring by a rheumatologist, as disease activity can change over time

The most effective prevention strategy combines adequate protein intake (1.0-1.2 grams per kilogram daily), comprehensive micronutrient supplementation from day one, gradual dose titration to moderate the rate of weight loss, maintaining a caloric floor, and baseline blood work to identify and correct pre-existing deficiencies

Hypothesis and Theory