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In step-b), Fmoc-Ser(tBu)-Tyr(tBu)-Leu-Glu(OtBu)-Gly-OH (fragment- 3) was condensed with peptide obtained from step-a) in presence of a coupling agent to obtain Fmoc-Ser(tBu)-Tyr(tBu)- Leu-Glu(OtBu)-Gly-Gln(Trt)-Ala-Ala-Lys(C18 diacid mono-t-butyl-y-Glu(AEEA-AEEA)- OtBu)-Glu(OtBu)-Phe-Ile-Ala-Trp(Boc)-Leu-Val-Arg(pbf)-Gly-Arg (pbf)-Gly-OtBu