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The retro-orbital injection of the construct into Mecp2 G311A mutant mice successfully restored MeCP2 protein expression and function, prolonged survival, and improved respiratory function of the mice

[DOI] [PMC free article] [PubMed] [Google Scholar] 106.Bellin M.D., Beilman G.J., Sutherland D.E., Ali H., Petersen A., Mongin S., Kirchner V., Schwarzenberg S.J., Trikudanathan G., Freeman M.L., et al

The medium used to maintain the pellet was full chondrogenic medium (CM) consisted of DMEM (Gibco), 2 anti-anti (Gibco), 10 g/ml ITS (Thermo Fisher Scientific), 0.1 M Dexamethasone (Sigma-Aldrich, St

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The amount of active peptide in that volume depends entirely on the concentration of the reconstituted solution