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Wash-free production scale synthesis In preparation for testing the wash-free method at a production scale, we first investigated the 25 mmol scale deprotection and coupling conditions by synthesizing liraglutide at a research scale (0.1 mmol) in a 35 mL reaction vessel

The biological effects at these doses are subtle enough that you may not notice the inconsistency, but the variable exposure undermines the purpose of a controlled protocol

(October 2025)