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After centrifugation and thorough removal of the supernatant, the pellets were incubated for 1 h in 60 l staining solution (1% BSA and 0.5% Tween-20 in PBS) containing 3 l anti-BrdUFITC monoclonal antibody (BioLegend, 364104, clone 3D4)

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Carruthers, 2017) show no cumulative toxicity over a decade of treatment, no measurable atrophy of treated muscle beyond what reverses on discontinuation, and stable result reproducibility across product brands

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