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Monooxygenases act by sequentially transferring two electrons from NADH or NADPH to one of the two atoms of oxygen in O 2 , generating H 2 O from one oxygen atom and incorporating the other oxygen atom into an organic compound as a hydroxyl group (R-OH)

Strategies to increase half-life To design GLP-RAs with an increased circulation time, a broad array of half-life extension strategies has been utilized including sequential modification (Byetta and Adlyxin), attachment of a fatty-acid (Victoza and Ozempic), development of a polymer-based sustained release formulation (Bydureon), fusion with human serum albumin (Tanzeum), and fusion with the fragment crystallizable (Fc) region of a monoclonal antibody (Trulicity) (Figure 1)

Glycine is the principal amino acid present in collagen, which is the bodys predominant protein, and therefore plays a critical structural role in healthy skin, bones, joints, blood vessels and connective tissue (tendons, ligaments and cartilage)

GST is also a commonly used solubility tag, meaning it is added N-terminally to another protein to help keep it soluble (Figure 2)

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