The glycosylation of the mucins also provides a physical and electrochemical barrier as the sugars interact with moieties on the surface of bacteria and viruses to bind and hold them in place slowing diffusion rates and reducing the effectiveness of microbial motility (McAuley et al., 2017
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Major barriers to the clinical use of gene therapy, however, include transfection efficiency, the creation of long-term effects, the difficulty of controlling cell proliferation, ectopic transfection, disease transmission, patient-specific dose responses, immune reactions against viral proteins, possible side effects, and ethical issues [87, 88]
The mechanism of regulation of each isoforms by different stresses and developmental stages is yet to be completely understood
A few packages, however, are marked "hazardous" and must be neutralized by a specific safety team (glutathione) before they can leave the building