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ECG Recording ECGs were recorded continuously in deeply anesthetized rats for all three main leads by positioning stainless steel electrodes on all four limbs using an ECG monitor with a 2090 programmer (Medtronic, Minneapolis, MN, USA) connected to a Waverunner LT342 digital oscilloscope (LeCroy, Chestnut Ridge, NY, USA) before procedure, after ligation and therapy application, and before sacrifice

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TB 500 is a synthetic form of Thymosin Beta 4, known for its role in promoting actin production and supporting cellular regeneration throughout the body

Med Sci Monit 16(3):BR81-8 Sharma P, Maffulli N (2005) Basic biology of tendon injury and healing

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