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By activating transcription factors like NF-B and activator protein 1 (AP-1) and upregulating kinases like phosphatidylinositol 3-kinase (PI3K) and mitogen-activated protein kinases (MAPKs), ROS generation can cause pro-inflammatory responses (Chen and Kunsch 2004)

It is possibility that volatile compounds generated in the urine may be affected by the bacterial contamination from environments carried out the sampling and the laboratory conditions, but we suggest that the major cause of different MMB levels between the two subspecies is variation of their metabolic pathway for the MMB production rather than the contamination