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H 2 O 2 signaling leads to the release of Nrf2 from complexes with Kelch-like ECH-associated protein 1 (Keap1) and ubiquitin ligase Cullin 3 and into the nucleus, where it binds to antioxidant response elements (ARE) and activates the expression of its target gene GPX8, which in turn regulates the expression of ER calcium by regulating SERCA ( 2 O 2 signaling and inhibiting Nrf2 translocation to the nucleus, resulting in a decrease in GPX8, resulting in increased ER calcium content and signaling ( via GPX8, in the same study, after silencing Nrf2 in HeLa cells, GPX8 mRNA and protein levels also decreased, resulting in elevated ER and cytosolic calcium levels at rest and increased histamine-induced ER calcium release (Tokuda et al., 2019)

The expulsed chromatin, coated with antimicrobial proteins, forms NETs (neutrophil extracellular traps), able not only to trap pathogenic microorganisms, but also to propagate inflammation and to favour the induction of microclots following platelet binding (144, 145)

March 18, 2026 Key Takeaways: Glutathione is the body's primary antioxidant, produced naturally in the liver