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The crypt palette was suspended in Matrigel (Corning) on ice
For the extraction, methanol and internal standards were added, followed by incubation for 15 min at 30C on a thermal shaker (650 rpm, TS1 ThermoShaker, Biometra, Gttingen, Germany), centrifugation (21,000 x g, 10 min, 4C, Heraeus Fresco 21 Centrifuge, Thermo Scientific, Schwerte, Germany), evaporation, and centrifugation with methanol again
234 In tumor cells, the activation of intracellular signaling pathways enhances FA synthesis and LD accumulation, leading to senescence in effector T cells
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