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One model using indomethacin-induced intestinal lesions (published in European Journal of Pharmacology ) found BPC-157 administration restored claudin-1 and occludin expression to near-baseline levels within 72 hours

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Briefly, cells were maintained in growth medium (High-glucose Dulbeccos modified Eagles medium (DMEM) supplemented with 10% FBS, 2 mM L-glutamine, 100 U/ml penicillin, 100 mg/ml streptomycin (all from Thermo Fisher Scientific Waltham, MA, United States))

Betaine hydrochloride is a naturally occurring component found in foods such as beets, spinach, and whole wheat