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The supernatant containing soluble protein was incubated with nickelnitrilotriacetic acid (Ni-NTA) agarose beads in binding buffer (lysis buffer supplemented with 20 mM imidazole) for 4 hours at 4 C to allow affinity binding

Novo Nordisk spent years developing Rybelsus , the first oral GLP-1 drug, which is FDA-approved for diabetes treatment and can only be prescribed for weight loss off-label

Participants also experienced reductions in cardiometabolic risk factors and improvements in quality of life metrics

Evidence level: This benefit has both in vitro mechanistic data and clinical wound care applications, making it one of the most well-supported GHK-Cu effects