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More significant healing needs may require 8-12 weeks

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To generate Gpx4 -/- and Acsl4 -/- clones by CRISPR Cas9 gene editing, transfection was performed for 48 h followed by puromycin (Gibco, A1113803) selection for 10 days and subsequent seeding onto 96-well plates with one cell per well for expansion

GAPDH in the nucleus is involved in apoptosis, particularly neuronal apoptosis

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