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Evaluation of bee venom as a novel feed additive in fast-growing broilers

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The enzymatic reaction was terminated by adding 2 urea Buffer (150 mM Tris pH 6.8, 6 M urea, 6% SDS, 40% glycerol, 100 mM DTT, 0.1% bromophenol blue) and heating the samples at 65C for 5 minutes

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