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The qPCR protocol consisted of initial denaturation at 95 C for 30 s, 40 cycles of amplification at 95 C for 5 s, 60 C for 30 s, and a final melting curve stage

3 Mechanisms of gut microbiota involved in the occurrence and development of live cirrhosis The occurrence and development of liver cirrhosis are significantly influenced by the gut microbiota

GLP-1 receptor agonists are already well established for their role in glycemic control and cardiovascular risk reduction

Ethics declarations Ethics approval and consent to participate This work was conducted according to the Helsinki Declaration, Belmont Report, International Ethical Guideline for Biomedical Research Involving Human Subjects and Council of International Organizations of Medical Sciences

Mevalonate improves anti-PD-1/PD-L1 efficacy by stabilizing CD274 mRNA