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1 g per well of cell membrane was incubated with furimazine (1:1,000 dilution from stock) in assay buffer (1 HBSS, 10 mM HEPES, 0.1% (w/v) OVA, 1 P8340 protease inhibitor cocktail, 1 mM DTT and 0.1 mM PMSF, pH 7.4)

Finally, the large wound where the skin was excised is closed with staples or sutures

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Med Clin North Am 79(1):937 Kjems LL, Holst JJ, Vlund A, Madsbad S (2003) The influence of GLP-1 on glucose-stimulated insulin secretion: effects on beta-cell sensitivity in type 2 and nondiabetic subjects