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The mechanism underlying carnitine's anti-aging effects involves multiple pathways: restoration of mitochondrial cardiolipin content and membrane integrity, enhancement of fatty acid oxidation to reduce lipotoxic stress, reduction of protein oxidation and carbonyl formation, improvement of calcium homeostasis and cellular signaling, and activation of stress resistance pathways that promote cellular resilience

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Flow cytometry analysis The acquisition of flow cytometry events was achieved using a BD LSRFortessa TM X-20 Cell Analyzer with BD FACSDiva software (BD Biosciences, Franklin Lakes, USA)