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Under microscopic visualization, the cochlear capsules are carefully removed from the temporal bone using forceps and transferred to a new cell culture dish containing ice-cold PBS
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Ligand binding drives receptor autophosphorylation, IRS-1/IRS-2 adapter recruitment, and activation of two primary downstream cascades: PI3K/Akt/mTOR (governing protein synthesis, glucose uptake, and cell survival) and MAPK/ERK (regulating cell proliferation and differentiation)
The injury flare is sometimes called a "healing response" and is attributed to TB-500 reactivating the inflammatory cascade in previously quiescent tissue
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