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10.1007/s10853-021-06123-6 188 TangY.ChenL.ZhaoK.WuZ.WangY.TanQ

A phase 2 study of OSI-906 (linsitinib, an insulin-like growth factor receptor-1 inhibitor) in patients with asymptomatic or mildly symptomatic (non-opioid requiring) metastatic castrate resistant prostate cancer (CRPC)

Unless indicated otherwise, cystine-free medium was prepared by supplementing cystine-, methionine- and glutamine-free DMEM (Gibco, CA, USA) with 100 M methionine, 4 mM glutamine, 1 mM sodium pyruvate, 0.2% bovine serum albumin (BSA), 100 U/ml penicillin and 100 g/ml streptomycin

Samples were stained with LIVE/DEAD fixable violet dead cell stain (1 l ml 1

It was tentatively suggested that this hormone might enhance sulfate uptake in normal control rat serum, implying an increase in amino acid uptake or synthesis