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Cell were washed and placed in normal Ringers buffer (160 mM NaCl, 4.5 mM KCl, 2 mM CaCl 2 , 1 mM MgCl 2 , 10 mM HEPES, pH 7.4) or Low Ca 2+ Ringers where CaCl 2 was omitted and 1 mM Ethylenediaminetetraacetic acid (EDTA) added

GSH also protects the cell from xenobiotics and heavy metals through the formation of GSH S-conjugates and their subsequent export in the vacuole or directly out of the cell

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In a state of infection, such as bacterial vaginosis, mucosal immune cells release pro-inflammatory cytokines (IL-6, IL-8, TNF-) 89

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