To compare the levels of mRNAs for proteins involved in thiamine-associated metabolism across different experiments, they are normalized to the summarized transcripts of GAPDH+ACTB+TUBA1A, as described previously (149)
This illustrates a general principle of circadian system organization, namely that there is a hierarchical system with the SCN master clock entraining and coordinating peripheral and non-SCN tissue clocks via endocrine and neuronal signals
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