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The 28-day post-reconstitution window is a hard limit for most practical purposes

Towards disease-oriented dosing of rapamycin for longevity: does aging exist or only age-related diseases

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Suspensions were disrupted by Fast-Prep (6.5 m/s, 45 s) twice and samples were cooled on ice between the cycles

Maintain this for 1-2 weeks before week 1 begins