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In vitro epithelial infection model For our infections, the medium was removed and replaced with prewarmed fresh RPMI-1640 (or RPMI-1640 with L-glutamine but without glucose, catalog number 11879020, Thermo Fisher Scientific) without FBS, and supplemented with or without 10 mg/mL albumin

Electric field guidance: Corneal wounds generate electric fields (wound potential) providing directional cues for cell migration

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Inflammatory Response They modulate inflammatory cytokine profiles in the reproductive tract, downregulating proinflammatory mediators like TNF-, IL-6, and IL-1, and sometimes increasing anti-inflammatory cytokines such as IL-10