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Jego synteza zachodzi w dwch etapach z ktrych oba s zalene od adenozyno-5-trifosforanu (ATP) [7] : najpierw kwas L-glutaminowy i L-cysteina s przeksztacane przez enzym syntetaz -glutamylocysteinow-glutamylocystein nastpnie -glutamylocysteina czy si z glicyn za porednictwem enzymu syntetazy glutationowej (GS) tworzc glutation