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After centrifugation, 10 l of the supernatant were filtered through a Microcon YM-10 centrifugal filter device (Amicon), and analyzed by reversed-phase high-performance liquid chromatography on a C18 column (Atlantis 5 m, 4,6200 mm, Waters) as previously described [18]

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If you consistently use tretinoin for three months you can expect to have a reduction in acne breakouts, smoother and firmer skin, a more even complexion, enhanced skin elasticity and more refined pores

Internat J Womens Dermatol

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