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In TGR, and more generally in TrxRs, electron transfer from NADPH to the enzyme is fast and practically irreversible and so an actual ES complex (NADPH-TGR) is not significantly populated during the catalytic cycle 17

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This mechanism is vital for maintaining cellular redox balance, eliminating harmful reactive oxygen species, and regenerating oxidized glutathione disulfide (GSSG) [3, 5]

PMID 13271446