Calu-3 cells (human lung adenocarcinoma cells) and HEp-2 cells (human epidermoid cancer cells) were maintained at 37 C with 5% CO 2 in Dulbeccos Modified Eagle Medium (DMEM) containing 10% FCS, GlutaMax, and 100 g/mL penicillin-streptomycin
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Glutathione benefits It's the bodys master detoxifier and plays a key role in the liver in detoxification Glutathione is one of the most important and powerful antioxidants It plays a role in healthy immune function The molecule serves as the body's main detoxification compound It is shown can protect the cells by helping to neutralize free radicals Higher intakes of glutathione, found abundantly in avocado, have been associated with an ability to help neutralize oxidants and bind with cellular mutagens
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The characteristics of glutathione are the following: (a) discovered by Hopkins in 1921 [67], (b) synthesized intracellularly by glutamate-cysteine ligase and glutathione synthetase [68], (c) exists in reduced (GSH) and oxidized (GSSG) forms, maintaining cellular redox balance [69], (d) functions in melanogenesis inhibition through tyrosinase suppression and pheomelanin synthesis [70], and (e) oral bioavailability is enhanced through liposomal formulations, S -acetyl derivatives, or co-administration with precursors [71]