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Intracytoplasmic sperm injection (ICSI), a form of IVF in which a single mature sperm is injected into the cytoplasm of a mature oocyte in the laboratory, is the most widely used assisted reproductive technique in the world

After 16 hours, the medium was replaced by fresh antibiotics-containing medium

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FIGURE 4 FIGURE 5 To further identify the prognostic significance of GLP-1 signaling-related genes in pan-cancer, Kaplan-Meier analyses with the best cut-off value and Univariate Cox regression analyses were performed in patients from TCGA database

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