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The reduction reaction was catalysed by PDI at room temperature, and the resulting aggregation of reduced insulin B chains was measured at 620 nm

doi: 10.1038/s41598-021-84881-8 390 Perez-PerezSDominguez-MozoMIAlonso-GomezAMedinaSVillarrubiaNFernandez-VelascoJIet al

Potential for Significant Side Effects: While not everyone experiences severe side effects, gastrointestinal symptoms can be pronounced for some individuals

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The absence of gradual dose escalation likely amplified the drugs inhibitory effects on gastric motility, precipitating severe nausea, vomiting, and subsequent acute kidney injury due to dehydration