Apply the juice to your skin using a cotton ball, leave it on for 15 minutes, and rinse off
PLoS ONE 11 (2016)
doi: 10.1002/jsfa.3959
Beads were washed with low salt buffer (0.1% SDS, 1% Triton X100, 2 mM EDTA, 20 mM TrisHCl, pH 8, and 150 mM NaCl), high salt buffer (0.1% SDS, 1% Triton X100, 2 mM EDTA, 20 mM TrisHCl, pH 8, and 500 mM NaCl), LiCl buffer (250 mM LiCl, 1% NP40, 1% NaDOC, 1 mM EDTA, and 10 mM TrisHCl, pH 8), twice with TE buffer(10 mM TrisHCl, pH 8, and 1 mM EDTA), and finally with elution buffer (1% SDS and 100 mM NaHCO 3 )
Quintana-Cabrera, R, Fernandez-Fernandez, S, Bobo-Jimenez, V, Escobar, J, Sastre, J, Almeida, A, Bolaos, JP 2012, -Glutamylcysteine detoxifies reactive oxygen species by acting as glutathione peroxidase-1 cofactor, Nature Communications , vol