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Increased TSH fosters cAMP synthesis, which in turn initiates cAMP-dependent protein kinase signaling systems, to potentiate EGF-mediated cell proliferation, stimulating thyroid cell growth and promoting tumor cell proliferation, invasion and metastasis (8688)

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Cells were subsequently mounted with PermaFluor and visualized using an inverted fluorescence microscope (Nikon Eclipse Ti) at 20 or 40 magnification

Each species pool (SP2 and SP5) was further subfractionated into individual colonies, and all members were evaluated alone or in combination with the members of the other pool

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