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Microdosing approaches have gained popularity

In particular with the CRISPRa approach, the transcriptional activators that are fused to the dCas9 enzyme will increase the length of the construct, such that a combination of sgRNA and dCas9-transcriptional activators will be too large to load onto a single AAV vector

For people with insurance coverage, there are usually multiple hoops to jump through, and each insurance companys prior authorization requirements are different, says Allison Rhodes, MD, an internal medicine and obesity medicine physician at the Ohio State University Wexner Medical Center in Columbus

Disadvantages are: raising potassium, lowering BP (dizzy on standing, dehydrated), reversibly raising liver tests (need to monitor every 3 months)

Vegetarian capsules, Dicalcium phosphate