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Materials and methods Cell lines and culture conditions Cell lines used in the study: Immortalized conditional Gpx4 / mouse embryonic fibroblasts expressing Cre-ERt2 (MEF, male) [33] were previously generated [32] with the CRISPR activation system [77] and a mouse Ms4a15 CRISPR guide (Supplementary Table 5) for overexpression, Calu-1 (gift from Brent Stockwell), HEK293T (fetal, ATCC Cat# CRL-3216), H1975 (female, ATCC Cat# CRL-5908)

The molecular and metabolic landscape of iron and ferroptosis in cardiovascular disease

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doi: 10.3389/fphar.2022.919181 203 XueB.LiH.GuoM.WangJ.XuY.ZouX.et al