International Journal of Plant Based Pharmaceuticals, 2 (2),190-195
To quantify SNAP/FLAG-hGLP-1R association with clathrin, circular ROIs surrounding individual well defined puncta of fluorescence (5-50 per cell) were selected on CLC-GFP images and transferred to the corresponding SNAP ones using a Fiji macro and average fluorescence per pixel was measured in a central circle (c) of diameter 5 pixels (0.55 m) at each puncta location, in a surrounding annulus (a) with an outer diameter of 7 pixels (0.77 m), and in an area not containing any cell as background (bg)
Cryer PE, Axelrod L, Grossman AB, Heller SR, Montori VM, Seaquist ER, et al
10.3760/cma.j.issn.0376-2491.2019.35.018 23
These species are generated as incidental outputs of various chemical reaction networks within systems and can induce structural and functional alterations in molecular assemblies, including nucleic acid polymers