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Transfection of Cyp2E1 [145] or induction of Cyp2E1 by dimethyl sulfoxide (DMSO) [139], can restore at least in part the metabolic capacity of HepG2 cells but the overall gene expression profile of HepG2 cells shows low similarity to primary human hepatocytes [146, 147]

The assumption of proportional hazards was evaluated with the generalized Schoenfeld approach on the TriNetX platform, with aHRs recalculated for specific time frames if initial assumptions were not met

To reduce variation and get a clearer picture of the effects of LC supplementation, especially on managing cardiovascular risk, future research should focus on larger, well-designed RCTs with more specific patient groups

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Melatonin is a naturally-occurring hormone that is also available in supplement form