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Expression of F-SNIP1 variants was induced with doxycycline (1 mg/1 ml of media) 18 h prior harvesting cells

The FLAG-tagged catalytically inactive IpaH1.4 C368A mutant and the substrate-binding LRR domain of IpaH1.4 were individually overexpressed in HEK293F cells, and then were enriched and pulled down via anti-FLAG beads

Vitamin K2 protects PC12 cells against A (1-42) and H2O2-induced apoptosis via p38 MAP kinase pathway

Answer: Yes, its safe to use daily

The ALT positive U2OS cells were included as a positive reference control, and C-circles were presented as percentages relative to the U2OS cell line (Henson et al