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The hypothesis that GSSG functions as the oxidant for class III peroxidases during ROS production in apoplast, however, is not consistent with previous observations that a GSH peak was not observed in a bean apoplastic fluid before and after an oxidative burst and glutathione was less effective for the production of reactive oxygen species than free cysteine in an in vitro assay 37,38

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The population was plated on solid YP20D medium containing 15 g/L agar and incubated at 30 C for 48 h

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