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K1 cells were again treated with various concentrations of RSL3 (0.0053 M), and Western blot analysis was performed

Another danger is the possibility of bacterial contamination during the manufacturing process

This doesn't interfere with the anesthesia's effectiveness during the operation, but it can support the "clean-up" process afterward

Furthermore, different studies employ varied sequencing methods (e.g., ITS1, ITS2, or 18S rRNA) and rely on fungal reference databases (e.g., UNITE, ITSoneDB) that are continuously updated 107,108 , which may introduce biases in species classification, lead to underdetection of low-abundance taxa, and complicate direct comparisons across studies