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Additional research considerations include characterizing whether the enhanced membrane permeability of the acetylated form alters tissue distribution in in vivo models, whether the modified charge distribution affects receptor or chromatin binding dynamics, and whether the extended half-life requires adjusted concentration protocols in cell culture systems

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According to one study [110], lung tissues from COPD patients exhibit ferroptosis due to hypermethylation of the CpG region of the Nrf2 promoter, which in turn prevents expression of Nrf2/GPX4

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