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The tissue sections were incubated overnight at 4 C with rabbit polyclonal anti-cleaved caspase-3 antibody (1:100, AB3623 Merck Millipore, Darmstadt, Germany) and rabbit polyclonal anti-GFAP antibody (1:200, ab7260, Abcam, Cambridge, UK)

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69 With only one intracerebroventricular injection of GLP-1 during the follicular phase, the LH levels in this phase were increased, boosting the progesterone levels in the luteal phase of the estrous cycle in female rats, which increased the number of fetuses implanted and born