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At the end of all incubations, the hECs were washed three times with ice-cold PBS containing 0.1% BSA, once with acid stripping buffer (glycine 50 mM, NaCl 100 mM, pH 3) to remove the non-internalized, but cell surface-attached nanocarriers, and finally with PBS

Several of these metabolites have already been explored as candidate biomarkers and therapeutic targets in preclinical studies [165]

Elevated immunoglobulin G4 levels in patients with thyroid eye disease and their clinical implications