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Then, sections were incubated overnight in a humidified chamber at 4C with primary antibody against rat anti-mouse neutrophil (Serotec), rat anti-mouse F4/80 (Serotec), mouse anti-nitrotyrosine (Santa Cruz), mouse anti-4-HNE (Abcam)
You can read more about this here: A review of adverse effects from IV glutathione
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