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In some embodiments, the polymer is carbomer or alginate

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The following day they were washed three times with 1 PBS followed by incubation at room temperature with secondary antibodies (Invitrogen/Fisher Scientific, Anti-Rabbit Alexa Fluor 647, Goat Polyclonal, Cat# A-21245, RRID:AB_2535813, dilution: 1:500) in 2% NGS in 1 PBS for one hour in the dark

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Its synthesis depends on GH