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26S proteasome activity assay Briefly, pulverized liver samples (20 mg aliquot) were homogenized in 26 S proteasome lysis buffer (50 mM Tris, 150 mM sodium chloride (NaCl), 1 mM ethylenediaminetetraacetic acid (EDTA), 5 mM magnesium chloride (MgCl 2) [pH 7.5]) with a hand-held Potter-Elvehjem homogenizer

Side Effects: Excessive doses may cause neuromuscular weakness and, in severe cases, respiratory arrest, particularly when administered rapidly by infusion

However, there are no general dosage recommendations, for example from the German Nutrition Society

Real-world data back this up