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[DOI] [PMC free article] [PubMed] [Google Scholar] 52.Boucher DM, Pedersen RA

McCord, J.M., Keele, B.B

Melasma is caused by overactive melanocytes producing excess melanin, triggered by three main factors: UV exposure, hormonal changes (pregnancy, birth control, thyroid), and genetics

Postfixative was removed and cells were washed (five times for 1 min each) with 0.1 M sodium cacodylate buffer pH 7.4 containing 2 mM CaCl 2 on ice and then washed (five times for 1 min each) with cold double-distilled H 2 O (ddH 2 O) on ice

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