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The flow was generated by a pump reproducing pulsatile pressure patterns (Cucullo et al., in vivo , including a low permeability, TEER values 10 times higher than in transwells, and an enhanced expression of pump molecules
ICD-9-CM, International Classification of Diseases, Ninth Revision, Clinical Modification
CAT was assayed as described by Durner & Klessing 53 , and the activity was determined as a decrease in the absorbance at 240 nm for 1 min following the decomposition of H 2 O 2
The protein bands were confirmed by detecting the luminescence emitted by Clarity TM Western ECL Substrate (Bio Rad) using ChemiDoc TM XRS+ with Image Lab TM Software (Bio Rad)