Data-independent acquisition mass spectrometry For DUB analysis on the Evosep platform, ubiquitylome and proteome of P5091-treated iNeurons, and proteome of NACET- and vehicle-treated mouse brains, samples were loaded onto Evotips following the manufacturers instructions and using protocols adapted from 111
moreover, the use of different drugs varies across geographical locations
Lentivirus production Lentivirus was produced in HEK-293T cells which were transfected using Lipofectamine 3000 (Thermo Fisher Scientific, Waltham, MA, USA) with the plasmid of interest, a packaging plasmid psPAX2, a gift from Didier Trono (Addgene plasmid # 12260
Park, J., Lee, K., Kim, K
In 20 M SL treatment, LsMAX1 gene showed the highest expression level in both shoot and root tissue (Fig