The resin was then collected by centrifugation at 500 g for 10 min and washed in gravity flow column with 5 CVs of buffer containing 20 mM HEPES, pH 7.5, 100 mM NaCl, 10% (v/v) glycerol, 5 mM MgCl 2 , 1 mM MnCl 2 , 25 M TCEP, 0.1% (w/v) LMNG, 0.02% (w/v) CHS and 5 M peptide 20, followed by washing with 15 CVs of buffer containing 20 mM HEPES, pH 7.5, 100 mM NaCl, 10% (v/v) glycerol, 5 mM MgCl 2 , 1 mM MnCl 2 , 25 M TCEP, 0.03% (w/v) LMNG, 0.01% (w/v) GDN, 0.008% (w/v) CHS and 5 M peptide 20
doi: 10.1007/s00394-020-02233-0 49 FlorouPAnagnostisPTheocharisPChourdakisMGoulisDG
Lower mitochondrial efficiency can influence energy production, muscle function, cognitive performance, and physical resilience over time
60 ForstT.Uhlig-LaskeB.RingA.Graefe-ModyU.FriedrichC.HerbachK.et al (2010)
- Supports the safe elimination of toxins from the body, preventing damage from toxicity